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Proceedings Paper

LOSOM: phase relief imaging can be achieved with confocal system
Author(s): Tong Peng; Hao Xie; Yichen Ding; Yiqing Lu; Dayong Jin; Peng Xi
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Paper Abstract

We reported recently that laser oblique scanning optical microscopy (LOSOM) is able to obtain a relief image in transparent sample directly. To optimize the performance of LOSOM, the parameters such as numerical aperture, the distance between the specimen and the fluorescent medium and the pinhole size are investigated in this work. A beam blocker is introduced in light path which enhances dramatically the visualization of local phase difference.

Paper Details

Date Published: 11 December 2012
PDF: 6 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531C (11 December 2012); doi: 10.1117/12.2000809
Show Author Affiliations
Tong Peng, Peking Univ. (China)
Xi'an Univ. of Technology (China)
Hao Xie, Peking Univ. (China)
Yichen Ding, Peking Univ. (China)
Yiqing Lu, Macquarie Univ. (Australia)
Dayong Jin, Macquarie Univ. (Australia)
Peng Xi, Peking Univ. (China)


Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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