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Proceedings Paper

Differences in activity profile of bacterial cultures studied by dynamic speckle patterns
Author(s): E. E. Ramírez-Miquet; I. Otero; D. Rodríguez; J. G. Darias; A. M. Combarro; O. R. Contreras
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Paper Abstract

We outline the main differences in the activity profile of bacterial cultures studied by dynamic laser speckle (or biospeckle) patterns. The activity is detected in two sorts of culture mediums. The optical setup and the experimental procedure are presented. The experimentally obtained images are processed by the temporal difference method and a qualitative assessment is made with the time history of speckle patterns of the sample. The main differences are studied after changing the culture medium composition. We conclude that the EC medium is suitable to detect the E. coli bacterial presence in early hours and that Mueller Hinton agar delays some additional hours to make possible the assessment of bacteria in time.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871P (22 February 2013); doi: 10.1117/12.2000576
Show Author Affiliations
E. E. Ramírez-Miquet, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)
I. Otero, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)
D. Rodríguez, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)
J. G. Darias, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)
A. M. Combarro, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)
O. R. Contreras, Ctr. de Aplicaciones Tecnologicas y Desarrollo Nuclear (Cuba)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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