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Proceedings Paper

Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy
Author(s): Yang Pu; Rui Tang; Jianpeng Xue; W. B. Wang; Baogang Xu; Duanwen Shen; Sharon Bloch; Mingzhou Zhou; S. Achilefu; R. R. Alfano
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Paper Abstract

The clinical diagnosis of most cancers is based on evaluation of histology microscopic slide to view the size and shape of cellular nuclei, and morphological structure of tissue. To achieve this goal in vivo and in deep tissue, near infrared (NIR) dyes-bovine serum albumin (BSA) and immunoglobulin G (IgG) conjugates were synthesized. The spectral study show that the absorption and fluorescence of the dye-conjugates are in the “tissue optical window” between 650 nm and 1100 nm. The internalization and pinocytosis of the synthesized compound were investigated in cell level using fluorescence microscopy to obtain the optimal concentration and staining time scale.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871Z (22 February 2013); doi: 10.1117/12.2000080
Show Author Affiliations
Yang Pu, The City College of New York (United States)
Rui Tang, Washington Univ. in St. Louis (United States)
Jianpeng Xue, Washington Univ. in St. Louis (United States)
China Pharmaceutical Univ. (China)
W. B. Wang, The City College of New York (United States)
Baogang Xu, Washington Univ. in St. Louis (United States)
Duanwen Shen, Washington Univ. in St. Louis (United States)
Sharon Bloch, Washington Univ. in St. Louis (United States)
Mingzhou Zhou, Washington Univ. in St. Louis (United States)
S. Achilefu, Washington Univ. in St. Louis (United States)
R. R. Alfano, The City College of New York (United States)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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