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Proceedings Paper

Lethal photosensitization of Helicobacter species
Author(s): Charles E. Millson; Michael Wilson; Alexander J. MacRobert; Wendy Thurrell; Peter Mlkvy; Claire Davies; Stephen G. Bown
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Paper Abstract

Helicobacter pylori (H. pylori) is associated with a large number of gastroduodenal disorders. Clearance of the bacteria has been shown to benefit patients with duodenal ulcers, gastric ulcers, and certain rare types of gastric tumors. Broad-spectrum antibiotics are the mainstay of current treatment strategies but side-effects, poor compliance, and drug resistance limit their usefulness. We sensitized H. pylori with toluidine blue, haematoporphyrin derivative, aluminum disulphonated phthalocyanine, methylene blue or protoporphyrin IX prior to exposure to low-power laser light from either a gallium aluminum arsenide laser or a helium neon gas laser. All 5 sensitizers caused reductions of greater than 1000-fold in the number of viable bacteria. Light alone had no effect and only HpD caused a significant decrease in bacterial numbers without laser light. Next, we sensitized H. mustelae on explanted ferret gastric mucosa (ex vivo) with the same sensitizers and exposed them to light from a copper vapor pumped dye laser tuned appropriately. MB caused significant reductions in bacterial counts. Successful lethal photosensitization of Helicobacter pylori both in vitro and ex vivo raises the possibility of a local method for eradicating the bacteria, especially as the bacteria are only found in those parts of the upper gastrointestinal tract that are accessible to the endoscope.

Paper Details

Date Published: 12 January 1995
PDF: 7 pages
Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); doi: 10.1117/12.199142
Show Author Affiliations
Charles E. Millson, Univ. College London Medical School (United Kingdom)
Michael Wilson, Eastman Dental Institute for Oral Health Care Sciences (United Kingdom)
Alexander J. MacRobert, Univ. College London Medical School (United Kingdom)
Wendy Thurrell, Whittington Hospital (United Kingdom)
Peter Mlkvy, Univ. College London Medical School (United Kingdom)
Claire Davies, Univ. College London Medical School (United Kingdom)
Stephen G. Bown, Univ. College London Medical School (United Kingdom)


Published in SPIE Proceedings Vol. 2325:
Photodynamic Therapy of Cancer II
Daniel Brault; Giulio Jori; Johan Moan; Benjamin Ehrenberg, Editor(s)

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