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Proceedings Paper

Characterization of endogenous fluorophores by picosecond laser fluorescence spectroscopy
Author(s): Wolfgang Hoehne; Werner Schramm; Mathias Nittka; Heinz Detlef Kronfeldt
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Paper Abstract

By time resolved fluorescence spectroscopy in the psec range the fluorescence behavior of flavines as important endogenous fluorophores was investigated. The substances were examined under various conditions (e.g. pure solutions and cellular suspensions; different buffer systems and pH values). Particular attention was dedicated to the properties of these coenzymes under in situ conditions. The results were applied to test numerical methods for characterization of complex mixtures of fluorophores and for discrimination of different states of flavines.

Paper Details

Date Published: 4 January 1995
PDF: 7 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198735
Show Author Affiliations
Wolfgang Hoehne, Forschungsinstitut fuer Molekulare Pharmakologie (Germany)
Werner Schramm, Forschungsinstitut fuer Molekulare Pharmakologie (Germany)
Mathias Nittka, Technische Univ. Berlin (Germany)
Heinz Detlef Kronfeldt, Technische Univ. Berlin (Germany)


Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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