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Proceedings Paper

Evaluation of changes in the NADH level between carcinogenic and normal tissue samples by use of fluorescence spectroscopy
Author(s): Volkmar Betz; Herbert Schneckenburger; H. P. Alleroeder; Gerhard W. Sybrecht; Joerg-Uwe Meyer
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Paper Abstract

The metabolism of cancer cells frequently shows higher glycolytic activity than normal tissue. This glycolytic activity can result in changes of the NAD+/NADH equilibrium and variations of NAD+$ and NADH levels. The objective of this study has been to monitor changes in NADH fluorescence spectra of cancerous and normal bronchial tissue. Spectra were taken from in- vitro samples of normal and carcinogenic bronchial tissue in a microspektrofluorometric setup and in a spectrofluorometer, the latter giving the Emission-Excitation-Matrix (EEM of the tissue fluorescence. Two numerical approaches were taken to determine changes in the NADH spectra. (1) Graphical comparison of normalized emission spectra shows a blue shift of the maximum for carcinogenic tissue. There is a good correspondence between the form of the spectrum and the spectrum of LDH-bound NADH. (2) Numerical analysis of the EEM from freeze slices showed an increased signal in the case of normal tissue and a decreased one in the case of cancerous samples in the spectral range that is typical for NADH.

Paper Details

Date Published: 4 January 1995
PDF: 8 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198733
Show Author Affiliations
Volkmar Betz, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
H. P. Alleroeder, Universitaetskliniken Homburg (Germany)
Gerhard W. Sybrecht, Universitaetskliniken Homburg (Germany)
Joerg-Uwe Meyer, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)


Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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