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Proceedings Paper

Fluorescence spectroscopy for colon cancer diagnosis
Author(s): George I. Zonios; Robert M. Cothren; Joseph Arendt; Jun Wu; James M. Crawford; Jacques Van Dam; Ramasamy Manoharan; Michael S. Feld
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Paper Abstract

Laser-induced fluorescence spectroscopy is a promising technique for detecting colonic dysplasia in vivo but, at present, the biological basis for the success of the method is poorly understood, and little information is provided as to the morphological/molecular origin of tissue fluorescence. We present a methodology for establishing this, taking as a starting point a recently completed prospective laser-induced fluorescence (LIF) clinical study of colonic polyps. The method is based on a morphological model of tissue fluorescence with three components: the intrinsic lineshapes of the fluorophores, the spatial distributions of their intensities, and the optical parameters of tissue. We measure these using fluorescence microspectroscopy, microscopic imaging and tissue optics, respectively. The model predicts the features of the clinical spectra, and quantifies the respective intrinsic and architectural contributions. The results can be inverted to extract biological parameters from the spectra, and used to select optimal excitation wavelength(s) and guide probe design. Implications to detection of nonpolypoid dysplasia are discussed.

Paper Details

Date Published: 4 January 1995
PDF: 5 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198726
Show Author Affiliations
George I. Zonios, Massachusetts Institute of Technology (United States)
Robert M. Cothren, Cleveland Clinic Foundation (United States)
Joseph Arendt, Cleveland Clinic Foundation (United States)
Jun Wu, Massachusetts Institute of Technology (United States)
James M. Crawford, Brigham and Women's Hospital (United States)
Jacques Van Dam, Brigham and Women's Hospital (United States)
Ramasamy Manoharan, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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