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Proceedings Paper

Time-gated spectroscopy of intrinsic fluorophores in cells and tissues
Author(s): Herbert Schneckenburger; Michael H. Gschwend; Ruediger J. Paul; Herbert G. Stepp; Kai Rick; Volkmar Betz; Wolfgang S. L. Strauss
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Paper Abstract

Based on novel time-resolving methods, the autofluorescence of saccharomyces, cultivated endothelial cells and biopsy specimens of human bladder was measured. The emission was found to be composed by the coenzymes NADH (free and protein-bound) and flavins with a concentration ratio of 100:1 between free NADH and flavin molecules. The fluorescence intensity of free NADH appeared to be a measure of the respiratory function. In addition, epithelial and connective tissues could be distinguished by the intensity ratio at 435 and 460 nm, which may be affected by the relation of bound and free NADH, but also by the extracellular fluorescence of elastin.

Paper Details

Date Published: 4 January 1995
PDF: 9 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198719
Show Author Affiliations
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
Michael H. Gschwend, Univ. Ulm (Germany)
Ruediger J. Paul, Heinrich-Heine-Univ. (Germany)
Herbert G. Stepp, Klinikum Grosshadern (Germany)
Kai Rick, Klinikum Grosshadern (Germany)
Volkmar Betz, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg M.D.; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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