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Proceedings Paper

Fiber optical sampling of NADH concentration in guinea pig hearts during ischemia
Author(s): Thomas Bocher; Juergen Beuthan; Olaf Minet; Isabella Schmitt; B. Fuchs; Gerhard J. Mueller
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Paper Abstract

A new method to detect and quantify ischemic states of tissue is presented. One important indicator of the metabolic and ischemic status of a cell is the concentration ratio of NADH : NAD+. The use of time-resolved laser- induced fluorescence techniques (LIF) in combination with previous biochemical measurements of NADH (Nicotineamide Adenine Dinucleotide) concentrations in tissue offer the possibility of quantitative NADH detection. The essential of the new method is a double-rescaling procedure of the fluorescence signal taking into account both the optical limitations of the device and the individually and locally varying optical tissue properties which are the scattering coefficient (mu) s, the absorption coefficient (mu) a and the anisotropy factor g. All the data presented in this paper were acquired from experiments with in vivo guinea pig hearts.

Paper Details

Date Published: 4 January 1995
PDF: 11 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198717
Show Author Affiliations
Thomas Bocher, Laser-Medizen-Zentrum GmbH (Germany)
Juergen Beuthan, Laser-Medizen-Zentrum GmbH (Germany)
Olaf Minet, Laser-Medizen-Zentrum GmbH (Germany)
Isabella Schmitt, Laser-Medizen-Zentrum GmbH (Germany)
B. Fuchs, Laser-Medizen-Zentrum GmbH (Germany)
Gerhard J. Mueller, Laser-Medizen-Zentrum GmbH (Germany)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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