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Proceedings Paper

Fluorescence spectroscopy and imaging of pH in vivo using a dual emission fluorophore (C-SNAFL-1)
Author(s): Serge R. Mordon; Jean-Marie Devoisselle; Sylvie Soulie-Begu
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Paper Abstract

Fluorescent intracellular pH indicators have been widely applied in recent years to monitor changes in intracellular pH. More recently, we have proposed the use of dual-excitation pH-fluorescent indicators (5,6-CF: 5,6- carboxyfluorescein or BCECF: 2',7'-bis-carboxyethyl-5,6-carboxy-fluorescein) to monitor pH on animals. This study aimed to evaluate a dual-emission fluorophore (C-SNAFL-1: 5' (and 6') carboxy-seminaphthofluorescein) for pH monitoring in-vivo. In that case, one excitation wavelength and two emissions are chosen to give a pH-dependent emission intensity ratio.

Paper Details

Date Published: 4 January 1995
PDF: 9 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198713
Show Author Affiliations
Serge R. Mordon, INSERM (France)
Jean-Marie Devoisselle, Univ. de Montpellier I (France)
Sylvie Soulie-Begu, Univ. de Montpellier I (France)


Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging

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