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Proceedings Paper

Histochemical analysis of human coronary artery using near-infrared Raman spectroscopy
Author(s): James F. Brennan; Tjeerd J. Roemer; Yang Wang; Maryann Fitzmaurice; Robert S. Lees; John R. Kramer; Michael S. Feld
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Paper Abstract

We are developing a method to quantitatively analyze the biochemical composition of human coronary artery in situ using near-infrared Raman spectroscopy. Samples of normal artery (intima/media and adventitia) and noncalcified and calcified plaque from coronary arteries, obtained from explanted recipient hearts during heart transplantation, were illuminated with 830 nm excitation light from a CW Ti:sapphire laser. Raman spectra were collected in seconds using a spectrograph and a cooled, deep-depletion CCD detector, and calibration and background corrections were made. Artery samples in different stages of atherosclerosis exhibited distinct spectral features, providing clear histochemical indicators for characterizing the type and extent of the lesion. Spectra were analyzed by means of a Raman biochemical assay model to determine the relative weight fractions of cholesterols, triacylglycerol, proteins and calcium minerals. Such information, when obtained clinically, promises to be useful in diagnosing and studying human atherosclerosis, its progression and response to drug therapy.

Paper Details

Date Published: 4 January 1995
PDF: 5 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198710
Show Author Affiliations
James F. Brennan, Massachusetts Institute of Technology (United States)
Tjeerd J. Roemer, Cleveland Clinic Foundation (Netherlands)
Yang Wang, Massachusetts Institute of Technology (United States)
Maryann Fitzmaurice, Case Western Reserve Univ. (United States)
Robert S. Lees, Harvard Univ. and Massachusetts Institute of Technology (United States)
John R. Kramer, Cleveland Clinic Foundation (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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