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Proceedings Paper

Fluorescence excitation and emission spectra of ALA-induced protoporphyrin IX in normal and tumoral tissue of the human bladder
Author(s): Martin Forrer; Thomas M. Glanzmann; Jerome C. Mizeret; Daniel Braichotte; Georges A. Wagnieres; Hubert van den Bergh; Patrice Jichlinski; Hans-Juerg Leisinger
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Paper Abstract

In vivo spectrofluorometric analysis represents a tool to obtain information about fluorophore distribution in tissue. Based on a Peltier-cooled CCD we designed a fluorescence excitation and emission spectrograph which allows to obtain tissue spectra endoscopically and in a clinical environment. Clinical studies were performed on patients with positive cytology or tumor recurrence in the urinary bladder. Patients received a 50 ml instillation of 3% ALA solution at pH 5.5 during 3 to 4 hours and underwent a normal white light cystoscopic examination together with light induced fluorescence photodetection at 5 to 8 hours after the beginning of the instillation. Local fluorescence measurements with a single fiber were performed before photodetection. These showed fluorescence ratios between tumor and normal tissue of 1.5 to 20 with the strongest ratios for exophytic papillary tumors. Fluorescence excitation between 380 nm and 450 nm revealed that the higher Protoporphyrin IX (PPIX) signal on tumor tissue is accompanied by a decrease of the autofluorescence at the emission wavelength of 500 nm.

Paper Details

Date Published: 4 January 1995
PDF: 5 pages
Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); doi: 10.1117/12.198705
Show Author Affiliations
Martin Forrer, Ecole Polytechnique Federale de Lausanne (Switzerland)
Thomas M. Glanzmann, Ecole Polytechnique Federale de Lausanne (Switzerland)
Jerome C. Mizeret, Ecole Polytechnique Federale de Lausanne (Switzerland)
Daniel Braichotte, Ecole Polytechnique Federale de Lausanne (Switzerland)
Georges A. Wagnieres, Massachusetts General Hospital (Switzerland)
Hubert van den Bergh, Ecole Polytechnique Federale de Lausanne (Switzerland)
Patrice Jichlinski, CHUV Hospital (Switzerland)
Hans-Juerg Leisinger, CHUV Hospital (Switzerland)


Published in SPIE Proceedings Vol. 2324:
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres, Editor(s)

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