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Photodynamic therapy of contractile arterial smooth muscle cells in vivo using aluminium disulphonated phthalocyanine: implications for angioplasty induced intimal hyperplasia
Author(s): Isaac Nyamekye; Jean R. McEwan; Alexander J. MacRobert; Christopher C. R. Bishop; Stephen G. Bown
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Paper Abstract

Photodynamic therapy (PDT) of proliferative vascular smooth muscle cells (SMC) reduces intimal hyperplasia. We asses the effects of PDT on contractile SMC In normal arteries using aluminum disulphonated phthalocyanine (AlS2Pc) sensitization. Rats sensitized with intravenous AlS2Pc underwent carotid artery fluorescence measurement. More rats had carotid artery irradiation with different laser energies 30 minutes after sensitization with AlS2Pc 5.0 mg/kg and sacrificed at 3 days. Additional rats were irradiated with 50 J/cm2 after sensitization with different AlS2Pc doses and sacrificed at 3 and 14 days. Histological analysis was by light microscopy. Rats treated with PDT and sacrificed at 3 and 6 months were also analyzed. The results show AlS2Pc induced high and dose dependent fluorescence within the arterial media. All PDT light doses (50 - 250 J/cm2) produced arterial SMC depletion. In contrast, PDT sensitizer doses produced complete cell depletion only at high doses of AlS2Pc. These treated arteries were acellular after 3 days. At 14 days the media remained acellular but the endothelial lining regenerated. The arterial media remained acellular even at 6 months -- however, no hemorrhage, thrombosis or aneurysm formation was seen. Arterial PDT causes media contractile SMC depletion in vivo and the artery retains its structural and functional integrity.

Paper Details

Date Published: 23 December 1994
PDF: 7 pages
Proc. SPIE 2327, Medical Applications of Lasers II, (23 December 1994); doi: 10.1117/12.197580
Show Author Affiliations
Isaac Nyamekye, The Rayne Institute and Middlesex Hospital (United Kingdom)
Jean R. McEwan, Univ. College London Medical School (United Kingdom)
Alexander J. MacRobert, The Rayne Institute (United Kingdom)
Christopher C. R. Bishop, Middlesex Hospital (United Kingdom)
Stephen G. Bown, The Rayne Institute (United Kingdom)

Published in SPIE Proceedings Vol. 2327:
Medical Applications of Lasers II
Stephen G. Bown; J. Escourrou; Frank Frank; Herbert J. Geschwind; Guilhem Godlewski; Frederic Laffitte; Hans H. Scherer, Editor(s)

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