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Proceedings Paper

Optical trapping and manipulation of single cells and motile microorganisms by laser diode radiation
Author(s): Carlo Frediani; Cesare Ascoli; S. Lucia; P. Verkerk; L. Guidoni; A. Fioretti; Ennio Arimondo
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Paper Abstract

Electromagnetic fields, emitted by laser sources, have been utilized in recent years for controlling the position and velocity of atoms, ions and microscopic neutral particles. In 1987 Ashkin has shown for the first time that cells too can be trapped by using a focused beam of laser radiation. The trapping is due to the interation between the electric dipole induced by the laser electric field in the cell and the electric field itself. In order to maximize the trapping effect and to avoid damage to the cells caused by excessive heating, the laser wavelength must be far from the absorption bands for both the cells and the solution where cells are kept, usually water. Our preliminary experiments, utilizing a 100 mW laser diode at 850 nm with suitable focusing, show that also free swimming (up to 100 micrometers /s) protozoa (Dunaliella salina) can be easily trapped, without apparent damage. The experimental set-up and the experiments on motile micro-organisms are presented. Possible biomedical applications are discussed.

Paper Details

Date Published: 22 December 1994
PDF: 6 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197506
Show Author Affiliations
Carlo Frediani, Istituto di Biofisica (Italy)
Cesare Ascoli, Istituto di Biofisica (Italy)
S. Lucia, Istituto di Biofisica (Italy)
P. Verkerk, Univ. di Pisa (Italy) and Univ. Pierre et Marie Curie (France)
L. Guidoni, Univ. di Pisa (Italy)
A. Fioretti, Univ. di Pisa and Scuola Normale Superiore (Italy)
Ennio Arimondo, Univ. di Pisa (Italy)


Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

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