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Proceedings Paper

Laser microtreatment for genetic manipulations and DNA diagnostics by a combination of microbeam and photonic tweezers (laser microbeam trap)
Author(s): Karl-Otto Greulich; Shamci Monajembashi; D. Celeda; N. Endlich; Holger Eickhoff; Carsten Hoyer; G. Leitz; Gerd Weber; J. Scheef; H. Rueterjans
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Paper Abstract

Genomes of higher organisms are larger than one typically expects. For example, the DNA of a single human cell is almost two meters long, the DNA in the human body covers the distance Earth-Sun approximately 140 times. This is often not considered in typical molecular biological approaches for DNA diagnostics, where usually only DNA of the length of a gene is investigated. Also, one basic aspect of sequencing the human genome is not really solved: the problem how to prepare the huge amounts of DNA required. Approaches from biomedical optics combined with new developments in single molecule biotechnology may at least contribute some parts of the puzzle. A large genome can be partitioned into portions comprising approximately 1% of the whole DNA using a laser microbeam. The single DNA fragment can be amplified by the polymerase chain reaction in order to obtain a sufficient amount of molecules for conventional DNA diagnostics or for analysis by octanucleotide hybridization. When not amplified by biotechnological processes, the individual DNA molecule can be visualized in the light microscope and can be manipulated and dissected with the laser microbeam trap. The DNA probes obtained by single molecule biotechnology can be employed for fluorescence in situ introduced into plant cells and subcellular structures even when other techniques fail. Since the laser microbeam trap allows to work in the interior of a cell without opening it, subcellular structures can be manipulated. For example, in algae, such structures can be moved out of their original position and used to study intracellular viscosities.

Paper Details

Date Published: 22 December 1994
PDF: 9 pages
Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); doi: 10.1117/12.197505
Show Author Affiliations
Karl-Otto Greulich, Institut fuer Molekulare Biotechnologie Postfach (Germany)
Shamci Monajembashi, Institut fuer Molekulare Biotechnologie Postfach (Germany)
D. Celeda, Institut fuer Molekulare Biotechnologie Postfach (Germany)
N. Endlich, Ruprecht-Karls-Univ. (Germany)
Holger Eickhoff, Institut fuer Molekulare Biotechnologie Postfach (Germany)
Carsten Hoyer, Institut fuer Molekulare Biotechnologie Postfach (Germany)
G. Leitz, Ruprecht-Karls-Univ. (Germany)
Gerd Weber, Institut fuer Pflanzenzuechtung (Germany)
J. Scheef, Institut fuer Molekulare Biotechnologie Postfach (Germany)
H. Rueterjans, Institut fuer Molekulare Biotechnologie Postfach (Germany)


Published in SPIE Proceedings Vol. 2328:
Biomedical Optoelectronic Devices and Systems II
Nathan I. Croitoru; Norbert Kroo; Mitsunobu Miyagi; Riccardo Pratesi; Juergen M. Wolfrum, Editor(s)

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