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Proceedings Paper

Dynamic light scattering study of the effect of Mg2+ and ATP on synthetic myosin filaments
Author(s): Satoru Fujime; Sei-ichi Takayama
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Paper Abstract

The dynamic light scattering (DLS) method provides us with information about the apparent diffusion coefficient, Dapp, as well as the static scattering intensity, Is. For long but thin rods with length L and diameter d (i.e., KL >> 1 but Kd << 1, K being the length of the scattering vector), the dependence on L and d of Dapp is quite different from that of Is. By means of DLS were studied synthetic myosin filaments of vertebrate skeletal muscle in solution at pH 8.3. It appeared that Mg2+ ions induced lengthening and thickening of the filaments whereas ATP (and ADP) induced shortening (depolymerization) of the filaments. When ATP was added to the filament preparation in the presence of Mg2+ ions, thinning of the filaments (or splitting into subfilaments) occurred prior to shortening (depolymerization).

Paper Details

Date Published: 2 January 1995
PDF: 12 pages
Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); doi: 10.1117/12.197462
Show Author Affiliations
Satoru Fujime, Yokohama City Univ. (Japan)
Sei-ichi Takayama, Yokohama City Univ. (Japan)

Published in SPIE Proceedings Vol. 2370:
5th International Conference on Laser Applications in Life Sciences
Pavel A. Apanasevich; Nikolai I. Koroteev; Sergei G. Kruglik; Victor N. Zadkov, Editor(s)

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