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Proceedings Paper

Study of structure and functional states of alcohol oxidase by surface-enhanced Raman spectroscopy
Author(s): Nataliya D. Strekal; Sergei A. Maskevich; Irina M. Artsukevich; Leonid N. Kivach; Ivan P. Chernikevich
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Paper Abstract

The SERS spectra of alcohol oxidase purified from Pichia Pastoris and Candida Boidinii adsorbed on silver electrode were obtained. The effect of stabilization of protein by NaN3 was studied by SERS of flavin adenine dinucleitide released from flavoprotein near the Ag surface. The dissociation of falvin from flavoprotein in N3-anion from and in neutral form independence on functional state of protein has been supposed. The association of protein oligomers and the peculiarities of quaternary structure are discussed as a reason of different SERS behavior of alcohol oxidase from various different sources.

Paper Details

Date Published: 2 January 1995
PDF: 5 pages
Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); doi: 10.1117/12.197441
Show Author Affiliations
Nataliya D. Strekal, Univ. of Grodno (Belarus)
Sergei A. Maskevich, Univ. of Grodno (Belarus)
Irina M. Artsukevich, Institute of Biochemistry (Belarus)
Leonid N. Kivach, Univ. of Grodno (Belarus)
Ivan P. Chernikevich, Institute of Biochemistry (Belarus)


Published in SPIE Proceedings Vol. 2370:
5th International Conference on Laser Applications in Life Sciences
Pavel A. Apanasevich; Nikolai I. Koroteev; Sergei G. Kruglik; Victor N. Zadkov, Editor(s)

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