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Proceedings Paper

3-D representation of microscopic specimens using confocal microscopy and digital image processing
Author(s): Kjell Carlsson
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Paper Abstract

In this paper a review is first given of how confocal fluorescence microscopy in combination with digital image processing can be utilized to present three-dimensional images of microscopic specimens. Examples of different display techniques are given as well as data on typical computing speeds when using a SUN 386i workstation. In the latter part of the paper we discuss signal quality and factors limiting the thickness of the specimen volume that can be studied. Multiple-wavelength registration is also described, as well as a method for eliminating detector "cross-talk" when recording two fluorophores with overlapping emission spectra.

Paper Details

Date Published: 1 May 1990
PDF: 13 pages
Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); doi: 10.1117/12.19542
Show Author Affiliations
Kjell Carlsson, Royal Institute of Technology (Sweden)


Published in SPIE Proceedings Vol. 1245:
Biomedical Image Processing
Alan Conrad Bovik; William E. Higgins, Editor(s)

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