Share Email Print

Proceedings Paper

Digital image processing of confocal microscopic images of the eye
Author(s): Barry R. Masters
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

This paper describes the formation and the processing of images from ocular tissue. Submicron optical serial sections of the cornea and the ocular lens were obtained using both the laser scanning confocal microscope and the Nipkow disk Tandem scanning microscope. The laser scanning confocal microscope used a photomultiplier tube as the detector. The real-time tandem scanning confocal light microscope used a cooled charge-coupled device (CCD) as the detector. Both confocal imaging systems are compared. The laser scanning confocal microscope used Kalman averaging to reduce the noise of the images. The real-time tandem scanning confocal microscope used a cooled CCD to integrate the image for 5 seconds in order to reduce image noise. The sample was a live, enucleated rabbit eye. The cornea and the ocular lens are almost transparent and have extremely low contrast. The images obtained with the two confocal systems show sub-micron resolution in the image plane. These confocal light microscope provide high resolution, high contrast images of living ocular tissue. The image quality of the resulting confocal images rivals that obtained from electron microscope of fixed, stained, and coated tissue specimens. Examples are given of simple digital image processing operations to alter the image quality.

Paper Details

Date Published: 1 May 1990
PDF: 18 pages
Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); doi: 10.1117/12.19538
Show Author Affiliations
Barry R. Masters, Georgia Institute of Technology (United States)

Published in SPIE Proceedings Vol. 1245:
Biomedical Image Processing
Alan Conrad Bovik; William E. Higgins, Editor(s)

© SPIE. Terms of Use
Back to Top