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Proceedings Paper

Thin-film microelectric arrays for amperometric enzyme biosensors with electrochemically synthesized glucose oxidase-polyaniline membrane
Author(s): Sergei V. Dzyadevich; Victor K. Rossokhaty; Nataly Shram; Alexander A. Shul'ga; Alexey P. Soldatkin; Vitaly I. Strikha
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Paper Abstract

An amperometric glucose biosensor was fabricated by the electrochemical polymerization of aniline onto a gold electrodes in presence of glucose oxidase in phosphate buffer solution, pH 7.0. Aniline is easily polymerized forming a thin film, which adheres tightly on the electrodes surface. During the electropolymerization process the enzyme was entrapped into the polyaniline film being able to catalyze the hydrolysis of glucose. The experiments were performed to determine the optimal condition for polyaniline-glucose oxidase film preparation. Glucose can be determined by the biosensor in the concentration range 10-4 M to 2 X 10-2 M. The linearity of the biosensor response was observed from 2 X 10-4 M to 6 X 10-3 M glucose, which demonstrated that the internal diffusion of substrates and products of reaction through the polyaniline layer to the electrodes surface was the main limiting factor controlling the response value. The method of electropolymerization was found to have several advantage in comparison with other approaches especially for further mass manufacturing of the biosensors.

Paper Details

Date Published: 26 October 1994
PDF: 6 pages
Proc. SPIE 2364, Second International Conference on Thin Film Physics and Applications, (26 October 1994); doi: 10.1117/12.190723
Show Author Affiliations
Sergei V. Dzyadevich, Shevchenko Kiev Univ. (Ukraine)
Victor K. Rossokhaty, Shevchenko Kiev Univ. (Ukraine)
Nataly Shram, Shevchenko Kiev Univ. (Ukraine)
Alexander A. Shul'ga, Shevchenko Kiev Univ. (Ukraine)
Alexey P. Soldatkin, Institute of Molecular Biology and Genetics (Ukraine)
Vitaly I. Strikha, Shevchenko Kiev Univ. (Ukraine)

Published in SPIE Proceedings Vol. 2364:
Second International Conference on Thin Film Physics and Applications
Shixun Zhou; Yongling Wang; Yi-Xin Chen; Shuzheng Mao, Editor(s)

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