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Proceedings Paper

Three-dimensional reconstruction of ocean chlorophyll distributions from underwater serial sectioned fluorescence images
Author(s): Andrew W. Palowitch; Jules S. Jaffe
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Paper Abstract

An underwater optical serial sectioning system has been developed to measure in-situ three- dimensional distributions of biological particles. The technique involves scanning a thin plane of laser light sequentially through a range of distances parallel to the imaging plane of a digital CCD camera. Images of induced fluorescence in the sequentially illuminated planes are recorded. A computer based inverse program is then used to reconstruct three-dimensional chlorophyll (alpha) distributions from the plane images. The inverse method compensates for attenuation of the fluorescence excitation and emission throughout the volume and converts camera image plane intensity values to mg Chl (alpha) /m3. Graphic display of the composite data set with spatial resolution of 1 cm3 in a 20 X 20 X 20 cm volume is presented in a three-dimensional rendered volume. Chl (alpha) concentration discrimination of 0.1 mg Chl (alpha) /m3 over the range of 0.1 to 2.0 mg Chl (alpha) /m3 has been demonstrated in the laboratory with the optical serial sectioning system.

Paper Details

Date Published: 26 October 1994
PDF: 11 pages
Proc. SPIE 2258, Ocean Optics XII, (26 October 1994); doi: 10.1117/12.190103
Show Author Affiliations
Andrew W. Palowitch, Scripps Institution of Oceanography (United States)
Jules S. Jaffe, Scripps Institution of Oceanography (United States)

Published in SPIE Proceedings Vol. 2258:
Ocean Optics XII
Jules S. Jaffe, Editor(s)

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