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Proceedings Paper

Simultaneous image and point spread function reconstruction for 3D light microscopy
Author(s): Timothy J. Holmes; Santosh Bhattacharyya; Joshua A. Cooper; David K. Hanzel; Vijaykumar Krishnamurthi; Wen-Chieh Lin; Badrinath Roysam; Donald H. Szarowski; James N. Turner
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Paper Abstract

Blind deconvolution algorithms are being developed for reconstructing (deblurring) 2D and 3D optically sectioned light micrographs, including widefield fluorescence, transmitted light brightfield and confocal fluorescence micrographs. The blind deconvolution concurrently reconstructs the point spread function (PSF) with the image data. This is important because it obviates the necessity to measure the PSF. The PSF measurement is esoteric and sometimes impossible and it thereby will hinder wide routine biological and clinical usage. The iterative algorithms are primarily based on a stochastic model of the physics of fluorescence quantum photons and the optimization criterion of maximum likelihood estimation (MLE), as extended from precursory nuclear medicine MLE algorithms. The algorithm design is mostly model based, although it contains some non- model-based components which have important practical benefits.

Paper Details

Date Published: 30 September 1994
PDF: 10 pages
Proc. SPIE 2302, Image Reconstruction and Restoration, (30 September 1994); doi: 10.1117/12.188054
Show Author Affiliations
Timothy J. Holmes, Rensselaer Polytechnic Institute and New York State Dept. of Health (United States)
Santosh Bhattacharyya, Rensselaer Polytechnic Institute (United States)
Joshua A. Cooper, Rensselaer Polytechnic Institute (United States)
David K. Hanzel, Molecular Dynamics, Inc. (United States)
Vijaykumar Krishnamurthi, Rensselaer Polytechnic Institute and Carnegie Mellon Univ. (United States)
Wen-Chieh Lin, Rensselaer Polytechnic Institute and New York State Dept. of Health (United States)
Badrinath Roysam, Rensselaer Polytechnic Institute and New York State Dept. of Health (United States)
Donald H. Szarowski, New York State Dept. of Health (United States)
James N. Turner, Rensselaer Polytechnic Institute and New York State Dept. of Health (United States)


Published in SPIE Proceedings Vol. 2302:
Image Reconstruction and Restoration
Timothy J. Schulz; Donald L. Snyder, Editor(s)

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