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Proceedings Paper

Three-dimensional reconstruction of myenteric neurons of the guinea pig
Author(s): Manachem Hanani; Leonid Ermilov; Vered Louzon; Philip Schmalz; Steven M. Miller; Joseph H. Szurszewski
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Paper Abstract

Neurons in the myenteric plexus have been studied for many years but their 3D structure is largely unknown. We used a laser scanning confocal microscope (LSCM) to obtain serial, optical sections of these cells and ANALYZE software to process the images into 3D. The 3D reconstructions showed that the neurons had a variety of shapes in cross sections, unlike the previously presumed flat shape. The short processes of Dogiel type I cells that have been considered to be very flat appeared to have a nearly circular cross section. The processes of the neurons lay in a single narrow area and thus appeared not to make vertical connections in their parent ganglia. The surface areas and volumes were calculated for both the cell bodies and processes. These data have not been available from conventional microscopy and they demonstrate the power of this method for analyzing neuronal morphology.

Paper Details

Date Published: 9 September 1994
PDF: 6 pages
Proc. SPIE 2359, Visualization in Biomedical Computing 1994, (9 September 1994); doi: 10.1117/12.185235
Show Author Affiliations
Manachem Hanani, Hebrew Univ. (Israel)
Leonid Ermilov, Mayo Clinic and Foundation (United States)
Vered Louzon, Hebrew Univ. (Israel)
Philip Schmalz, Mayo Clinic and Foundation (United States)
Steven M. Miller, Mayo Clinic and Foundation (United States)
Joseph H. Szurszewski, Mayo Clinic and Foundation (United States)

Published in SPIE Proceedings Vol. 2359:
Visualization in Biomedical Computing 1994
Richard A. Robb, Editor(s)

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