Share Email Print
cover

Proceedings Paper

In vivo fluorescence spectroscopy and imaging of ALA-induced endogenous porphyrins in skin after Er:YAG ablation of human stratum corneum
Author(s): Karsten Koenig; Herbert Schneckenburger; Wolf-Henning Boehncke; Raimund Hibst
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Limited regions of human stratum corneum were removed by laser ablation using an Er:YAG laser. Immediately after this procedure, an ointment containing 5-aminolevulinic acid (ALA) was applied topically to the laser-treated and surrounding skin. The time-dependent ALA- induced biosynthesis of protoporphyrin IX was measured by fluorescence detection. Fluorescence in the red spectral region was found to occur in the ablated skin regions only. Time-resolved measurements showed the formation of long-lived fluorophores (16 ns) indicating the presence of ALA-induced monomeric porphyrin. Naturally occurring fluorophores (NAD(P)H, flavins, collagen, elastin) possess shorter fluorescence decay times. Therefore, time-gated measurements in the nanosecond region enable the specific detection of ALA-stimulated porphyrin fluorescence by choosing an appropriate time-window. In addition, detection of backscattered excitation light can be avoided. High-contrast video images of ALA-incubated fluorescent areas were obtained using this novel imaging technique.

Paper Details

Date Published: 7 September 1994
PDF: 7 pages
Proc. SPIE 2128, Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV, (7 September 1994); doi: 10.1117/12.184901
Show Author Affiliations
Karsten Koenig, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (FRG) and Beckman Laser Institute (Germany)
Herbert Schneckenburger, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)
Wolf-Henning Boehncke, Univ. Ulm (Germany)
Raimund Hibst, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 2128:
Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV
R. Rox Anderson; R. Rox Anderson, Editor(s)

© SPIE. Terms of Use
Back to Top