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Proceedings Paper

In vivo fluorescence spectroscopy and imaging of ALA-induced endogenous porphyrins in skin after Er:YAG ablation of human stratum corneum
Author(s): Karsten Koenig; Herbert Schneckenburger; Wolf-Henning Boehncke; Raimund Hibst
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Paper Abstract

Limited regions of human stratum corneum were removed by laser ablation using an Er:YAG laser. Immediately after this procedure, an ointment containing 5-aminolevulinic acid (ALA) was applied topically to the laser-treated and surrounding skin. The time-dependent ALA- induced biosynthesis of protoporphyrin IX was measured by fluorescence detection. Fluorescence in the red spectral region was found to occur in the ablated skin regions only. Time-resolved measurements showed the formation of long-lived fluorophores (16 ns) indicating the presence of ALA-induced monomeric porphyrin. Naturally occurring fluorophores (NAD(P)H, flavins, collagen, elastin) possess shorter fluorescence decay times. Therefore, time-gated measurements in the nanosecond region enable the specific detection of ALA-stimulated porphyrin fluorescence by choosing an appropriate time-window. In addition, detection of backscattered excitation light can be avoided. High-contrast video images of ALA-incubated fluorescent areas were obtained using this novel imaging technique.

Paper Details

Date Published: 7 September 1994
PDF: 7 pages
Proc. SPIE 2128, Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV, (7 September 1994); doi: 10.1117/12.184901
Show Author Affiliations
Karsten Koenig, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (FRG) and Beckman Laser Institute (Germany)
Herbert Schneckenburger, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)
Wolf-Henning Boehncke, Univ. Ulm (Germany)
Raimund Hibst, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 2128:
Laser Surgery: Advanced Characterization, Therapeutics, and Systems IV
R. Rox Anderson; R. Rox Anderson, Editor(s)

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