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Proceedings Paper

Observation and stimulation of biological processes using excimer lasers
Author(s): Karl-Otto Greulich; Juergen M. Wolfrum
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Paper Abstract

Examples are given for the application o f high power excimer lasers in spectroscopy and processing of biological material. An excimer-laser pumped dye laser serves as light source for a pulsed UV Raman spectrometer which allows resonant Raman studies on nucleic acids. Experiments on the pH induced double helix formation of poly adenylic acid are described. By combining the excimer laser with a distributed feedback dye laser and a streak camera, a picosecond UV fluorescence spectrometer is built up .Tyrosine fluorescence lifetimes of selected tryptophan free peptides with up to 9 amino acids can be explained in a surprisingly simple way: only the directly neighbouring amino acid on the C-terminal side and only a few amino acids on the N-terminal side have an influence on the fluorecscence lifetime of these peptides. Besides spectroscopic applications, the excimer laser serves as light source for processing of biological material. For medical applications , high power UV Laser light has to be transmitted through light guides. A tapered light guide transmitting more than GW/cm2 is described. Microprocessing of biological material with accuracies of a few hundred nanometers can be performed when an excimer pumped dye laser is coupled into a microscope .The resulting UV laser microbeam can be used to introduce foreign genetic material into plant cells, tissues and subcellular organelles such as mitochondria and chioroplasts. Selected pairs of different cells can be fused in the UV laser microbeam under total microscopic control. Finally, one can microdissect human chromosomes and isolate DNA probes for the analysis of human disease.

Paper Details

Date Published: 1 June 1990
PDF: 12 pages
Proc. SPIE 1225, High-Power Gas Lasers, (1 June 1990); doi: 10.1117/12.18485
Show Author Affiliations
Karl-Otto Greulich, Ruprecht-Karls-Univ./Heidelberg (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ./Heidelberg (Germany)


Published in SPIE Proceedings Vol. 1225:
High-Power Gas Lasers
Petras V. Avizonis; Charles Freed; Jin J. Kim; Frank K. Tittel, Editor(s)

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