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Proceedings Paper

Effect of CO2 laser amputation on hydra regeneration
Author(s): L. De Petrocellis; Andrea Finizio; R. Minei; Pasquale Mormile; Giovanni Pierattini
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Paper Abstract

In order to investigate laser effects on biological specimens, Hydra, a coelenterate with high regeneration rate, was observed for ten days during regeneration after CO2 laser cutting. Control animals were cut with a razor blade immediately below the tentacle whorl under a dissecting microscope while they were in small glass petri dishes. They regenerated tentacles completely 8 to 10 days from the cutting. Hydra were cut in the same position with CO2 laser. As a first step, we studied the effect of the laser beam on the normal behaviour of hydra. For the cutting, we used four different power intensities: 0.5, 1.0, 1.5 and 2.0 W. At different power intensities the animals regenerated the tentacles. However in about 20% of the animals the amputation performed with 1 and 1.5 W originated a quicker regeneration of tentacles. No effect was observed on asexual reproduction of the polyps and therefore also no change of the bud index.

Paper Details

Date Published: 10 August 1994
PDF: 6 pages
Proc. SPIE 2097, Laser Applications, (10 August 1994); doi: 10.1117/12.183129
Show Author Affiliations
L. De Petrocellis, Istituto di Cibernetica del CNR (Italy)
Andrea Finizio, Istituto di Cibernetica del CNR (Italy)
R. Minei, Istituto di Cibernetica del CNR (Italy)
Pasquale Mormile, Istituto di Cibernetica del CNR (Italy)
Giovanni Pierattini, Istituto di Cibernetica del CNR (Italy)


Published in SPIE Proceedings Vol. 2097:
Laser Applications
Artur A. Mak, Editor(s)

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