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Proceedings Paper

Fluorescence lifetime imaging microscopy (FLIM) and its applications
Author(s): Xue Feng Wang; Ammasi Periasamy; Gerald W. Gordon; Pawel Wodnicki; Brian Herman
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Paper Abstract

Recent technological advances have provided an opportunity for the development of `fluorescence lifetime imaging microscopy' (FLIM). FLIM is an extremely important advance, as it allows for the first time, the sensitivity of the fluorescence lifetime to environmental parameters to be monitored in a spatial manner in single living cells. FLIM can be developed both on conventional and confocal fluorescence microscopes. Fluorescence lifetime detection can be performed using either time- or frequency-domain methods. In this paper, we report on the development of conventional and confocal FLIM systems currently underway in our laboratory. We also present three examples of current biological research projects in which we employ FLIM.

Paper Details

Date Published: 17 August 1994
PDF: 13 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182779
Show Author Affiliations
Xue Feng Wang, Univ. of North Carolina/Chapel Hill School of Medicine (United States)
Ammasi Periasamy, Univ. of North Carolina/Chapel Hill (United States)
Gerald W. Gordon, Univ. of North Carolina/Chapel Hill School of Medicine (United States)
Pawel Wodnicki, Univ. of North Carolina/Chapel Hill School of Medicine (United States)
Brian Herman, Univ. of North Carolina/Chapel Hill School of Medicine (United States)


Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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