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Proceedings Paper

One- and two-pulse theory of light quenching
Author(s): Jozef Kusba; Valery L. Bogdanov; Ignacy Gryczynski; Joseph R. Lakowicz
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Paper Abstract

We have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high-repetition rate lasers, a phenomenon we call 'light quenching'. We now describe some of the possible effects of light quenching on the time- resolved intensity and anisotropy decays of fluorophores. We show that light quenching can decrease or increase the time-zero anisotropy, and that the initial anisotropies can increase to unity under selected conditions. Quenching with time-delayed light pulses is predicted to result in oscillations in the frequency-domain intensity and anisotropy decays. The increasing available and use of pulsed laser sources of light offers the opportunity for a new class of one- two- or multiple-pulse time- resolved experiments.

Paper Details

Date Published: 17 August 1994
PDF: 14 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182737
Show Author Affiliations
Jozef Kusba, Univ. of Maryland/Baltimore School of Medicine (United States)
Valery L. Bogdanov, Univ. of Maryland/Baltimore School of Medicine (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)


Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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