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Proceedings Paper

Optimized streak-camera system: wide excitation range and extended time scale for fluorescence lifetime measurement
Author(s): Urs Graf; Christof Buehler; Michael Betz; Herbert Zuber; M. Anliker
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Paper Abstract

A new versatile system for the measurement of time-resolved fluorescence emission spectra of biomolecules is presented. Frequency doubling and tripling of a Ti:Sapphire laser allows excitation over a wide wavelength range. The influence of increasing the spectral resolution on the time resolution has been investigated. System performance can be optimized for best resolution in the spectral or time domain, respectively. System performance can be optimized for best resolution in the spectral or time domain, respectively. The currently achieved temporal resolution is 6 psec, and the best spectral resolution is 3 nm. Long fluorescence decays can be resolved with optimal time resolution by way of taking into account the flyback of the streak camera. With the system described, the core complex ((alpha) (beta) )3APCLC8.9 of the phycobilisome from the photosynthetic cyanobacteria Mastigocladus laminosus has been analyzed. Lifetime analysis clearly demonstrated the influence of the linker polypeptide on the phycobiliprotein complex and the identity of native and reconstituted complex.

Paper Details

Date Published: 17 August 1994
PDF: 7 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182729
Show Author Affiliations
Urs Graf, Swiss Federal Institute of Technology and Univ. of Zurich (Switzerland)
Christof Buehler, Swiss Federal Institute of Technology and Univ. of Zurich (Switzerland)
Michael Betz, Swiss Federal Institute of Technology (Switzerland)
Herbert Zuber, Swiss Federal Institute of Technology (Switzerland)
M. Anliker, Swiss Federal Institute of Technology and Univ. of Zurich (Switzerland)


Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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