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Proceedings Paper

Center for Fluorescence Spectroscopy: advanced studies of fluorescence dynamics, lifetime imaging, clinical sensing, two-photon excitation, and light quenching
Author(s): Joseph R. Lakowicz; Henryk M. Malak; Ignacy Gryczynski; Henryk Szmacinski; Jozef Kusba; Engin Akkaya; Ewald A. Terpetschnig; Michael L. Johnson
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Paper Abstract

The Center for Fluorescence Spectroscopy (CFS) is a multi-user facility providing state of the art time-resolved fluorescence instrumentation and software for scientists, whose research can be enhanced by such experimental data. The CFS is a national center, supported by the National Center for Research Resources Division of the National Institutes of Health, and in part by the National Science Foundation. Both time-domain (TD) and frequency- domain (FD) measurements (10 MHz to 10 Ghz) are available, with a wide range of excitation and emission wavelengths (UV to NIR). The data can be used to recover distances and site-to-site diffusion in protein, interactions between macromolecules, accessibility of fluorophores to quenchers, and the dynamic properties of proteins, membranes and nucleic acids. Current software provides for analysis of multi-exponential intensity and anisotropy decays, lifetime distribution, distance distributions for independent observation of fluorescence donors and acceptors, transient effects in collisional quenching, phase-modulation spectra and time-resolved emission spectra. Most programs provide for global analysis of multiple data sets obtained under similar experimental conditions. Data can be analyzed on-site by connection with the CFS computers through the internet. During six years of operation we have established scientific collaborations with over 30 academic and industrial groups in the United States. These collaborations have resulted in 63 scientific papers.

Paper Details

Date Published: 17 August 1994
PDF: 4 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182725
Show Author Affiliations
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Henryk M. Malak, Univ. of Maryland/Baltimore School of Medicine (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore School of Medicine (United States)
Henryk Szmacinski, Univ. of Maryland/Baltimore School of Medicine (United States)
Jozef Kusba, Univ. of Maryland/Baltimore School of Medicine (United States)
Engin Akkaya, Univ. of Maryland/Baltimore School of Medicine (United States)
Ewald A. Terpetschnig, Univ. of Maryland/Baltimore School of Medicine (United States)
Michael L. Johnson, Univ. of Maryland (United States)


Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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