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Proceedings Paper

Near-field fluorescence microscopy and spectroscopy: applications to single chromophores, single proteins, and photosynthetic membranes
Author(s): Sunney Xie; Errol V. Allen; Gary R. Holtom; Robert C. Dunn; Laurens Mets
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Paper Abstract

Near-field fluorescence microscopy combines nanometer resolution with the ability to conduct optical measurements with high sensitivity. To illustrate the potential of the near-field technique, we summarize recent results from our laboratory which include the fluorescence imaging of single dye molecules and single proteins and picosecond fluorescent lifetime measurements on intact photosynthetic membranes, all done at room temperature with nanometric spatial resolution.

Paper Details

Date Published: 17 August 1994
PDF: 13 pages
Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182708
Show Author Affiliations
Sunney Xie, Battelle Memorial Institute/Pacific Northwest Lab. (United States)
Errol V. Allen, Battelle Memorial Institute/Pacific Northwest Lab. (United States)
Gary R. Holtom, Battelle Memorial Institute/Pacific Northwest Lab. (United States)
Robert C. Dunn, Battelle Memorial Institute/Pacific Northwest Lab. (United States)
Laurens Mets, Univ. of Chicago (United States)


Published in SPIE Proceedings Vol. 2137:
Time-Resolved Laser Spectroscopy in Biochemistry IV
Joseph R. Lakowicz, Editor(s)

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