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Proceedings Paper

Near-IR biosensor for evanescent wave immunoassays
Author(s): Joel P. Golden; Lisa C. Shriver-Lake; Narasimhachari Narayanan; Gabor Patonay; Frances S. Ligler
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Paper Abstract

Based upon a biosensor design which utilizes standard fluorescent dyes (FITC, TRITC), a new device has been developed which incorporates a laser diode light source to excite novel near infrared (NIR) dyes. The purpose of switching to the NIR regime is to decrease the background fluorescence of biological samples and to decrease the size and power requirements of the biosensor. New dyes which fluoresce in the NIR have been conjugated to protein antigen and immunoassays performed. Assay results using excitation at 780 nm are shown.

Paper Details

Date Published: 27 July 1994
PDF: 5 pages
Proc. SPIE 2138, Longer Wavelength Lasers and Applications, (27 July 1994); doi: 10.1117/12.181363
Show Author Affiliations
Joel P. Golden, Naval Research Lab. (United States)
Lisa C. Shriver-Lake, Naval Research Lab. (United States)
Narasimhachari Narayanan, Georgia State Univ. (United States)
Gabor Patonay, Georgia State Univ. (United States)
Frances S. Ligler, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 2138:
Longer Wavelength Lasers and Applications
Gabor Patonay, Editor(s)

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