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Proceedings Paper

Laser-based blood cell measurements for the biological reconstruction of ionizing radiation doses using a novel technique for cytometry/biochemical reaction analysis
Author(s): Pavel G. Pleshanov; Richard G. Langlois; L. A, Azarov; D. A. Budayev; A. L. Ossipenkov; D. M. Shamayev
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Paper Abstract

A novel biodosimetry method based on red blood cell analysis by means of laser flow cytometry and specially designed monoclonal antibodies is used for reconstructing ionizing radiation doses for Chernobyl accident victims. In addition to analysis of the patient's blood samples, we are evaluating both spectroscopy procedures and laser-based flow techniques. It was shown that resonance energy transfer processes can take place among the two different dyes links to the spherical cell surface. This can affect the accuracy of rare mutant cell scoring. Also a new technique providing conventional flow cytometric analysis and kinetic measurements of the elementary stages of biochemical reactions is described.

Paper Details

Date Published: 21 July 1994
PDF: 12 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180807
Show Author Affiliations
Pavel G. Pleshanov, Institute of Biologically Active Substances (Russia)
Richard G. Langlois, Lawrence Livermore National Lab. (United States)
L. A, Azarov, Moscow Engineering Physics Institute (Russia)
D. A. Budayev, Moscow Engineering Physics Institute (Russia)
A. L. Ossipenkov, Moscow Engineering Physics Institute (Russia)
D. M. Shamayev, Moscow Engineering Physics Institute (Russia)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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