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Proceedings Paper

Image analysis of the blood cells for cytomorphodiagnostics and control of the effectiveness treatment
Author(s): Alexander V. Zhukotsky; Emmanuil M. Kogan; Victor F. Kopylov; Oleg V. Marchenko; O. A. Lomakin
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Paper Abstract

A new method for morphodensitometric analysis of blood cells was applied for medically screening some ecological influence and infection pathologies. A complex algorithm of computational image processing was created for supra molecular restructurings of interphase chromatin of lymphocytes research. It includes specific methods of staining and unifies different quantitative analysis methods. Our experience with the use of a television image analyzer in cytological and immunological studies made it possible to carry out some research in morphometric analysis of chromatin structure in interphase lymphocyte nuclei in genetic and virus pathologies. In our study to characterize lymphocytes as an image-forming system by a rigorous mathematical description we used an approach involving contaminant evaluation of the topography of chromatin network intact and victims' lymphocytes. It is also possible to digitize data, which revealed significant distinctions between control and experiment. The method allows us to observe the minute structural changes in chromatin, especially eu- and hetero-chromatin that were previously studied by genetics only in chromosomes.

Paper Details

Date Published: 21 July 1994
PDF: 5 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180806
Show Author Affiliations
Alexander V. Zhukotsky, Moscow Institute of Physicochemical Medicine (Russia)
Emmanuil M. Kogan, Moscow Institute of Physicochemical Medicine (Russia)
Victor F. Kopylov, Moscow Institute of Physicochemical Medicine (Russia)
Oleg V. Marchenko, Moscow Institute of Physicochemical Medicine (Russia)
O. A. Lomakin, Moscow Institute of Physicochemical Medicine (Russia)

Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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