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Proceedings Paper

Ultrasensitive fluorescence detection and maximum likelihood method
Author(s): Joerg Enderlein
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Paper Abstract

The maximum likelihood criterion is shown to be a powerful method for analyzing fluorescence detection data with small signal to noise ratios. Probability studies of the maximum likelihood criterion for supposed detection experiments of very small amounts of fluorescing molecules, down to a single molecule, are presented. In these studies the photokinetics of the molecules, their flow and diffusion, and the laser-beam geometry are taken into account. The efficiency of time-integrated and time-correlated single-photon counting methods are studied and compared.

Paper Details

Date Published: 21 July 1994
PDF: 10 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180805
Show Author Affiliations
Joerg Enderlein, BioQuant Ltd. (Germany)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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