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Proceedings Paper

New compact TCSPC apparatus based on sub 15 ps laserdiodes: preliminary results
Author(s): Rainer Erdmann; Gerald Kell; Wolfgang Becker; Edgar O. Klose
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Paper Abstract

The features and the behavior of a modular ultra-fast time correlated single photon counting (TCSPC) system are analyzed. Using a Ti-Sapphire laser as excitation source an instrumental response function (IRF) of less than 17 ps for the total system was achieved. Despite this excellent IRF time there are at present some problems in analyzing fluorescence decay curves with such an ultra-fast IRF because of increased influence of color shift, time-spatial relationship, and electronic noise. Furthermore, we developed a new compact experimental set-up for TCSPC measurements consisting of laserdiodes including generator DL 4000 with pulse width less than 15 ps and the PC plug-in electronic card SPC 300. Thus the financial and spatial expense for TCSPC systems can be extremely reduced. Besides the traditional single channel data acquisition the new electronics allow parallel detection and acquisition of up to 128 channels simultaneously. This offers a lot of new applications for the highly sensitive TCSPC technique.

Paper Details

Date Published: 21 July 1994
PDF: 7 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180798
Show Author Affiliations
Rainer Erdmann, GNF e.V. (Germany)
Gerald Kell, Humboldt Univ. (Germany)
Wolfgang Becker, Becker & Hickl Ltd. (Germany)
Edgar O. Klose, BioQuant Ltd. (Germany)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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