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Proceedings Paper

Room-temperature phosphorescence of metalloporphyrins and its application to immunoassay
Author(s): Alexander P. Savitsky; Gely V. Ponomarev; Oleg I. Lobanov; Nikita A. Sadovsky
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Paper Abstract

A novel type of highly efficient phosphorescent labels is proposed for time-resolved phosphorescent immunoassay (TR-PIA). The phosphorescent properties of palladium and platinum coproporphyrins and different derivatives of tetraphenylporphyrin were studied in deoxygenated micellar solution and at the dry surface in the presence of oxygen. Lifetime, quantum yield, and quantum efficiency in time-resolved mode of phosphorescence measurements for more than 20 compounds were determined. At the optimum condition the detection limit of Pd- or Pt-coproporphyrin of 10-13M may be achieved. The labels are thermodynamically and kinetically stable and insensitive to contamination of solutions and samples with ions of heavy metals. The measurement of room temperature phosphorescence of metalloporphyrin in micellar solution are rather simple and highly reproducible, the phosphorescent signal is stable for several hours and microtiter plates are suitable for micellar and dry surface measurements. Techniques have been developed for covalent and non-covalent labeling of proteins and oligonucletides with the phosphorescent label. The labeling proteins can be stored in the lyophilized state rather long and retain their immunological properties.

Paper Details

Date Published: 21 July 1994
PDF: 14 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180796
Show Author Affiliations
Alexander P. Savitsky, Institute of Biochemistry (Russia)
Gely V. Ponomarev, Institute of Biochemistry (Russia)
Oleg I. Lobanov, Institute of Biochemistry (Russia)
Nikita A. Sadovsky, Moscow State Univ. (Russia)

Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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