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Proceedings Paper

Diagnostics of physiological states of organism by means of fluorescent measurement of membrane potential of blood cells
Author(s): Tatyana V. Vengrus; Galina Zatsepina; Svetlana V. Egudina; Nikolay N. Goryunov; Olga V. Tarnopolskaya
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Paper Abstract

Average membrane potential (MP) of T-lymphocytes in different physiological states of mice were measured with the help of fluorescent probe anilinonaphtalin-8-sulphonate (ANS). We investigated thymocytes of healthy animals and animals in whose organisms inflammatory process (IP) or cancer (IC) was developed. It was shown that MP is different in different physiological states of mice and is 61 mV, 96 mV, and 112 mV in control, IP, and IC respectively. Kinetics of regulation of volume and MP of T-lymphocytes in different physiological states in hypotonic medium were also investigated. It was shown that the kinetics have different quasiperiodic characters in the case of healthy organism and in the case of IP. During development of IC T-lymphocytes lost their adaptability to hypotension. The disappearance of adaptability of T-lymphocytes beginning from the third day of development of IC represents an early test of cancer. The relationship between MP and surface potential of plasmatic membrane was also investigated.

Paper Details

Date Published: 21 July 1994
PDF: 7 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180784
Show Author Affiliations
Tatyana V. Vengrus, Moscow State Univ. (Russia)
Galina Zatsepina, Moscow State Univ. (Russia)
Svetlana V. Egudina, Moscow State Univ. (Russia)
Nikolay N. Goryunov, Moscow State Univ. (Russia)
Olga V. Tarnopolskaya, Moscow State Univ. (Russia)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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