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Proceedings Paper

Substrate-induced quenching-based measurement of glycerol
Author(s): Ashutosh Sharma; Nigel S. M. Quantrill
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Paper Abstract

Glycerol concentrations are measured using a novel photo reaction between an excited fluorophore and NADH, the latter being generated due to the reduction of NAD+ in an enzymatic reaction between glycerol dehydrogenase (GlDH) and glycerol. The results are treated using a modified Stern-Volmer type relation and at equilibrium, a SIQ quenching constant of 9.78 (+/- 2.05) M-1 is obtained for the substrate induced quenching of thionine by glycerol. The reported method was investigated over the glycerol concentration range of 0 - 100 mM and offers a detection limit of 0.4 mM.

Paper Details

Date Published: 21 July 1994
PDF: 5 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180781
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)
Nigel S. M. Quantrill, Cranfield Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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