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Proceedings Paper

Novel optical method for measuring D-glucose 6-phosphate
Author(s): Ashutosh Sharma; Nigel S. M. Quantrill
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Paper Abstract

A novel D-glucose 6-phosphate (G6P) assay is described that exploits the technique of substrate induced quenching. In a reaction between G6P and glucose 6-phosphate dehydrogenase (G6P-DH) the cofactor nicotinamide adenine dinucleotide (NAD+) is reduced to NADH. The latter being a quencher of thionine allows an indirect determination of the G6P concentration. A Stern-Volmer type analysis of the data for the investigated range of 0 - 1 mM G6P results in a quenching constant of 439 (+/- 37) M-1, with a detection limit of 9 (mu) M. A simple empirical model based on enzyme kinetics is presented for the overall G6P assay. Excellent correlation exists between the results predicted by the model and those obtained experimentally. Possibilities of employing the proposed G6P SIQ assay in multi-enzyme assays and in the construction of immunoassays is discussed.

Paper Details

Date Published: 21 July 1994
PDF: 7 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180780
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)
Nigel S. M. Quantrill, Cranfield Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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