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Proceedings Paper

Biodiagnostics with multiplex dyes
Author(s): Stefan Seeger; Jutta Arden-Jacob; Gerhard Deltau; Karl-Heinz Drexhage; Kyung-Tae Han; Malte Koellner; Ralph Mueller; Markus Sauer; Juergen M. Wolfrum
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Paper Abstract

A new bioanalytical concept that uses the characteristic fluorescence lifetimes of new multiplex dyes as an identification parameter is presented. Several dyes can be distinguished at the same wavelength by recognition of the fluorescence lifetimes. It is shown that the identification can be achieved in a capillary gel electrophoresis system in a few milliseconds with a misclassification rate of 10-4. As an example the simultaneous detection of two antibodies at one wavelength is presented.

Paper Details

Date Published: 21 July 1994
PDF: 12 pages
Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180774
Show Author Affiliations
Stefan Seeger, Ruprecht-Karls-Univ. Heidelberg (Switzerland)
Jutta Arden-Jacob, Univ. Gesamthochschule Siegen (Germany)
Gerhard Deltau, Univ. Gesamthochschule Siegen (Germany)
Karl-Heinz Drexhage, Univ. Gesamthochschule Siegen (Germany)
Kyung-Tae Han, Ruprecht-Karls-Univ. Heidelberg (Germany)
Malte Koellner, Ruprecht-Karls-Univ. Heidelberg (Germany)
Ralph Mueller, Ruprecht-Karls-Univ. Heidelberg (Germany)
Markus Sauer, Ruprecht-Karls-Univ. Heidelberg (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2136:
Biochemical Diagnostic Instrumentation
Robert F. Bonner; Gerald E. Cohn; Thomas M. Laue; Alexander V. Priezzhev, Editor(s)

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