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Proceedings Paper

Novel reduced nicotinamide adenine dinucleotide optical sensor
Author(s): Ashutosh Sharma
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Paper Abstract

A novel method for the fluorometric measurement of an analyte is described that is based on the anti-Stokes fluorescence of an indicator. The validity of the concept is demonstrated by developing a sensitive assay for NADH. Thionine, a thiazine dye, when excited using radiation of a wavelength 630 nm, emits a broad fluorescence, peaking at 620 nm. Similar to the Stokes fluorescence, this anti-Stokes' fluorescence of thionine is efficiently quenched by NADH. Stern-Volmer plots of the quenching data reveal a quenching constant of 0.7 X 103 M-1. The process of quenching is attributed to a photo-reaction between the excited thionine and NADH, resulting in NAD+.

Paper Details

Date Published: 28 July 1994
PDF: 4 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180770
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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