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Proceedings Paper

Novel fluorescence method for development of nicotinamide adenine dinucleotide and secondary biosensors
Author(s): Ashutosh Sharma
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Paper Abstract

Photon fusion is proposed as a new method for molecular sensor development. In a molecular system, higher wavelength radiation is absorbed, and a lower wavelength radiation is emitted that is quenched by an analyte. This concept is demonstrated by developing a sensitive assay for reduced nicotinamide adenine dinucleotide (NADH). Indicator benzopurpurin 4B, when excited at 458 nm, emits at 357 nm. This latter fluorescence is quenched extremely efficiently by NADH, with a half quenching concentration of 40 (mu) M and a Stern-Volmer constant of 25 X 103 M-1. The advantages of this method are outlined.

Paper Details

Date Published: 28 July 1994
PDF: 4 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180768
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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