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Proceedings Paper

Novel L-glutamate assay based on fluorescence quenching
Author(s): Ashutosh Sharma; Nigel S. M. Quantrill
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Paper Abstract

A novel optical procedure for measuring L-glutamate concentrations is described that exploits the quenching of thionine fluorescence by NADH, the latter being generated due to the reduction of NAD+ in an enzymatic reaction between L-glutamate dehydrogenase (GlDH) and L-glutamate. A substrate induced quenching (SIQ) constant of 660 (+/- 140) M-1 at 200 s is obtained. The reported method was investigated over the L- glutamate concentration range of 0 - 800 (mu) M and offers a detection limit of 6 (mu) M.

Paper Details

Date Published: 28 July 1994
PDF: 7 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180765
Show Author Affiliations
Ashutosh Sharma, Cranfield Univ. (United Kingdom)
Nigel S. M. Quantrill, Cranfield Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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