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Proceedings Paper

Single-mode tapered optical fiber loop immunosensor II: assay of anti-cholera toxin immunoglobulins
Author(s): Robert S. Marks; Zoe M. Hale; Myron M. Levine; C. R. Lowe; Frank P. Payne
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Paper Abstract

An evanescent wave immunoassay for cholera antitoxin immunoglobulins was performed using a single mode tapered optical fiber loop sensor. The transducer was silanized with 3- glycidoxypropyltrimethoxysilane and chemically modified to link covalently either cholera toxin B subunit or a synthetic peptide derived from it, CTP3. The sensor was exposed to seral fluids, obtained from human volunteers having been exposed to live virulent Vibrio cholerae 01 and shown to produce rice-water stools. Other toxins of interest, such as Clostridium botulinum toxin A, have been tested on similar systems. The bound unlabelled immunoglobulins were then exposed to a mixture of FITC-anti-IgG and TRITC-anti-IgA, without requirement for a separation step. The emanating fluorescent emissions of fluorescein and rhodamine, excited by the input laser light, were coupled back into the guided mode of the tapered fiber, and used to determine the concentrations of the complementary antigens.

Paper Details

Date Published: 28 July 1994
PDF: 9 pages
Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); doi: 10.1117/12.180747
Show Author Affiliations
Robert S. Marks, Univ. of Cambridge (United Kingdom)
Zoe M. Hale, Univ. of Cambridge (United Kingdom)
Myron M. Levine, Univ. of Maryland School of Medicine (United States)
C. R. Lowe, Univ. of Cambridge (United Kingdom)
Frank P. Payne, Univ. of Cambridge (United Kingdom)

Published in SPIE Proceedings Vol. 2131:
Biomedical Fiber Optic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir; Fred P. Milanovich, Editor(s)

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