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Proceedings Paper

Point fluorescence measurements of transformed tissues using 2-[1-hexyloxyethel]-2-devinyl pyropheophorbide-a
Author(s): Kinya Furukawa; David H. Crean; Alexander C. Kuebler; Thomas S. Mang; Harubumi Kato; Thomas J. Dougherty
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Paper Abstract

Recently, a new photosensitizer, 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-1 (HPPH) was developed for PDT which possesses more rapid clearance from skin and greater cytotoxicity per drug dose than Photofrin. The spectral characteristics of HPPH shows an absorption band at 665 nm (50,000 M-1cm(superscript -1 and peak emission at 680 nm. The aid of this study was to examine HPPH as a fluorescent diagnostic compound for the detection of transformed tissues using the in vivo fluorescence photodetector. The model of tissue transformation was the carcinogen (DMBA [9, 10-dimethyl-1, 2- benzanthracene])-induced premalignant and malignant lesions of the hamster buccal cheek pouch. The results demonstrated significant correlations between fluorescence levels and histological developments at all time points after injection. Time course studies of HPPH showed highest fluorescence readings at 48 hours after injection of 0.5 mg/kg HPPH (mild-moderate dysplasia, 0.35 +/- 0.17 volts; papillary disease with severe dysplasia, 0.58 +/- 0.33 volts; and squamous cell carcinoma, 1.04 $OM 0.32 volts). Therefore, it appears that HPPH may be a promising fluorophore for the detection of transformed tissues.

Paper Details

Date Published: 19 July 1994
PDF: 8 pages
Proc. SPIE 2133, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy III, (19 July 1994); doi: 10.1117/12.179980
Show Author Affiliations
Kinya Furukawa, Roswell Park Cancer Institute and Toyoko Medical College (Japan)
David H. Crean, Ontario Cancer Institute (Canada)
Alexander C. Kuebler, Roswell Park Cancer Institute (United States)
Thomas S. Mang, Roswell Park Cancer Institute (United States)
Harubumi Kato, Tokyo Medical College (Japan)
Thomas J. Dougherty, Roswell Park Cancer Institute (United States)


Published in SPIE Proceedings Vol. 2133:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy III
Thomas J. Dougherty, Editor(s)

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