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Proceedings Paper

Laser diffraction of RBC: the method and its pitfalls
Author(s): Rainer Bayer; Serdar Caglayan; Rainer Hofmann; Dieter Ostuni
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Paper Abstract

The method to determine red blood cell deformability by laser diffraction is presented. It combines ektacytometry (laser diffraction) with image analysis. Laser light is passed through erythrocyte suspensions, which are sheared in a Searle like viscometer. The laser diffraction patterns are photographed by a CCD camera and further analyzed by a computer. Flexibility is characterized by the quotient of minor and major axes of isointensity lines of the elliptically transformed diffraction patterns. The variation coefficient of the measurement is less than 0.5%.

Paper Details

Date Published: 28 June 1994
PDF: 8 pages
Proc. SPIE 2100, Cell and Biotissue Optics: Applications in Laser Diagnostics and Therapy, (28 June 1994); doi: 10.1117/12.179022
Show Author Affiliations
Rainer Bayer, Heinrich-Heine Univ. (Germany)
Serdar Caglayan, Heinrich-Heine Univ. (Germany)
Rainer Hofmann, Heinrich-Heine Univ. (Germany)
Dieter Ostuni, Heinrich-Heine Univ. (Germany)

Published in SPIE Proceedings Vol. 2100:
Cell and Biotissue Optics: Applications in Laser Diagnostics and Therapy
Valery V. Tuchin, Editor(s)

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