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Proceedings Paper

Picosecond fluorescence microscopy
Author(s): Motoyuki Watanabe; Musubu Koishi; Hirofumi Kan; Kenneth J. Kaufmann; Yutaka Tsuchiya
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Paper Abstract

A new picosecond time-resolved fluorescence microscope (PFM) has been developed for measuring and mapping the lifetime of laser excited fluorescence from a sample placed under a micro— scope. A compact laser diode is used to generate excitation pulses. The combination of a spectrograph and a streak camera performs time resolved fluorescence spectroscopy with a resolution of 40 ps ( 5 ps with deconvolusion). The lifetime and the intensity of laser excited fluorescence are analyzed b a computer and are displayed on a monitor as either a lifetime or intensity map.

Paper Details

Date Published: 1 April 1990
PDF: 8 pages
Proc. SPIE 1209, Picosecond and Femtosecond Spectroscopy from Laboratory to Real World, (1 April 1990); doi: 10.1117/12.17901
Show Author Affiliations
Motoyuki Watanabe, Hamamatsu Photonics KK (Japan)
Musubu Koishi, Hamamatsu Photonics KK (Japan)
Hirofumi Kan, Hamamatsu Photonics KK (Japan)
Kenneth J. Kaufmann, Hamamatsu Photonic Systems Corp. (United States)
Yutaka Tsuchiya, Hamamatsu Photonics KK (Japan)


Published in SPIE Proceedings Vol. 1209:
Picosecond and Femtosecond Spectroscopy from Laboratory to Real World
Keith A. Nelson, Editor(s)

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