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Proceedings Paper

Matrix metalloproteinase expression in excimer laser wounded rabbit corneas
Author(s): Taewon Hahn; Wallace Chamon; Yonja Akova; Walter J. Stark; William G. Stetler-Stevenson; Dimitri T. Azar
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Paper Abstract

This study was performed to obtain information about matrix metalloproteinase (MMP) expression in excimer-wounded corneas and to determine whether MMPs expression correlates with the depth of the ablation. 6-mm excimer keratectomy (60 or 180 micrometers ) was performed using the 193-mm ArF excimer laser on 12 NZW rabbits. Corneas treated with mechanical epithelial debridement and untreated corneas served as controls. Rabbits were killed at 20 and 30 hr after laser ablation. Zymography after SDS extraction was performed on regenerated central epithelium and the central stroma to determine MMPs expression. We observed enzymatic activity of a 92 KDa band in the epithelium of excimer-ablated corneas but not in that following debridement wounds and untreated controls. The expression of the 92 KDa MMP was most pronounced with the deeper excimer ablation. A 72 KDa band of enzymatic activity present in the stroma of all treated and control eyes was also seen in the epithelium of excimer-ablated corneas. These proteolytic enzymes may play an important role in wound healing and remodelling after excimer keratectomy.

Paper Details

Date Published: 23 June 1994
PDF: 5 pages
Proc. SPIE 2126, Ophthalmic Technologies IV, (23 June 1994); doi: 10.1117/12.178583
Show Author Affiliations
Taewon Hahn, Wilmer Eye Institute/Johns Hopkins Univ. School of Medicine (United States)
Wallace Chamon, Wilmer Eye Institute/Johns Hopkins Univ. School of Medicine (United States)
Yonja Akova, Wilmer Eye Institute/Johns Hopkins Univ. School of Medicine (United States)
Walter J. Stark, Wilmer Eye Institute/Johns Hopkins Univ. School of Medicine (United States)
William G. Stetler-Stevenson, National Cancer Institute (United States)
Dimitri T. Azar, Wilmer Eye Institute/Johns Hopkins Univ. School of Medicine (United States)


Published in SPIE Proceedings Vol. 2126:
Ophthalmic Technologies IV
Jean-Marie A. Parel; Qiushi Ren, Editor(s)

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