Share Email Print
cover

Proceedings Paper

Biological pigments as fluorescent labels for cytometry
Author(s): Diether J. Recktenwald; Barbara Prezelin; Chia H. Chen; John Kimura
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The use of protein-based biological pigments in combination with low molecular weight organic dye molecules allows the simultaneous detection of up to six independent components of a cell surface by antibody dependent fluorescence (immunofluorescence) excited with a single 488nm laser line. This paper describes the state of the art in multi-color immunofluorescence with 488nm Ar-ion laser excitation. It provides the data to design systems for specialized applications.

Paper Details

Date Published: 1 July 1990
PDF: 6 pages
Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); doi: 10.1117/12.17823
Show Author Affiliations
Diether J. Recktenwald, Becton Dickinson Immunocytometry Systems (United States)
Barbara Prezelin, Univ. of California/Santa Barbara (United States)
Chia H. Chen, Becton Dickinson Immunocytometry Systems (United States)
John Kimura, Becton Dickinson Immunocytometry Systems (United States)


Published in SPIE Proceedings Vol. 1206:
New Technologies in Cytometry and Molecular Biology
Gary C. Salzman, Editor(s)

© SPIE. Terms of Use
Back to Top